wiley tissue grinder

Bacteria detected in the honeybee Wiley Online Library
· Each sample was then washed three times with PBST and homogenized in 500 μl PBST using the Radnoti tissue grinder ( No. 440613 Monrovia CA). The total DNA was extracted with the Wizard ® Genomic DNA Purification kit (Promega Madison WI) according to
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Guanase activity in normal and neoplastic human tissue
Latapie tissue grinder and then homogenized in a Teflon pestle tissue grinder. Tissue sus- pensions were brought to 10 wet weight con- centration in 0.25M sucrose. Extracts were then centrifuged at 20 OOOXg at 5O (2. for 10 minutes and the supernatants were either assayed that day or
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wiley tissue grindercabaretzeewolde
Plant Tissue Grinder at Thomas ScientificPlant Tissue Grinder found in 2010 Geno/Grinder Thomas Wiley Mini-Mill Cutting Mill 1 ml Tissue Grinder Tapered Gnd AgileGrinder™ Portable Tissu wiley tissue grinder
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Tolerability and age-dependent toxicokinetics Wiley
· Fetuses were weighed and homogenized using a tissue grinder until a uniform homogenate was obtained. Stock solution of the internal standard methoxyurea was prepared in methanol at 1 mg/ml and diluted in acetonitrile to make a 10-μg/ml solution.
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Mitochondrial dysfunction in mut Wiley Online Library
· Tissue samples were homogenized in a 2-ml Tenbroeck tissue grinder (Wheaton Science Products Millville NJ USA) with T-Per ® (Pierce Biotechnology Rockford IL USA) tissue protein extraction buffer in the presence of a Halt™ (Pierce Biotechnology) protease inhibitor cocktail. Aliquots (20–40 µg) of clarified extracts were analyzed by
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Thomas Mill at Thomas Scientific
Dounce Tissue Grinder is supplied with two interchangeable pestles for large and small clearances. Packed in high-density polyethylene case with foam inserts. Complete kit includes one each of Thomas numbers 3432S50 S55 S70 S80 S90 and T30 Tissue Grinders.
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Summer autotrophy and winter heterotrophy in the
For Chl and zooxanthellae measurements coral tissue was detached from the skeleton using a Water PickH in 12 mL of 0.45-mm–filtered seawater. The slurry was homogenized using a Potter tissue grinder and a 2-mL subsample was taken for zooxanthellae density determination using the HistolabH 5.2.3 image analysis software (Rodolfo-Metalpa et al
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Laboratory Grinder at Thomas Scientific
Laboratory Grinder found in Laboratory Jar Mills Series 8000 Grindomix Denville Ultra EZgrind™ Tissue Homogenizer Package Thomas Wiley® Mini Cutting..
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Effect of fish oil and telmisartan Wiley Online Library
a tissue grinder (4°C) in 1–2 ml of phosphate-buffered saline. Then the homogenate was centrifuged at 3 000 rpm and the supernatant was used for determination of the tissue biochemical parameters. The right ovaries were subjected to histopathological and immuno-histochemical examination. 2.5 Determination of fasting blood glucose
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Coffee rust genome measured using Wiley Online Library
· The treatment in which the fungal material was ground 20 times with the pestle and tissue grindertogether with the nuclear isolation buffer OTTO-I (for 10 min) and staining buffer OTTO-II (for 20 min)proved to be optimal for the release of a sufficient quantity of intact and isolated nuclei from the urediniospores for stoichiometric
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Mitochondria from rat liver Method Wiley Online
tissue grinder. Do not use a ground glass homogenizer. Fill the homogenizer to 1 inch (2.5 cm) below the flare with solution B and make three gentle passes by hand with the pestle in a slow downward rocking motion rather than twisting or grinding motion. A more vigorous machine driven homogenization may
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Cytokinins in the ball gall of Wiley Online Library
· The larvae were extracted in cold 80 methanol plus 20 mg l −1 BHT in a 7-ml tissue grinder. 2 H 5 -trans Z and 2 H 5 -trans ZR (98 2 H 5 ) were added as internal standards to account for losses during extraction. The extraction procedure was as described for the quantitative plant tissue extracts except that a cellulose phosphate column
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Effect of the elapsed time between Wiley Online Library
· For glycoprotein release (also referred to as antigen retrieval) 40 mg of specimens were first homogenized in a Tapered Tissue Grinder (VWR Budapest Hungary) followed by the addition of 100 µL of RIPA buffer and 10 µL of 50 mM DTT to the homogenized samples and kept at 100˚C for 20 min then at 65˚C for 120 min.
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Tolerability and age-dependent toxicokinetics Wiley
· Fetuses were weighed and homogenized using a tissue grinder until a uniform homogenate was obtained. Stock solution of the internal standard methoxyurea was prepared in methanol at 1 mg/ml and diluted in acetonitrile to make a 10-μg/ml solution.
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ALGAL CELL DISRUPTION USING A PYREX TISSUE GRINDER Wiley
A motor‐driven Pyrex tissue grinder (PTG) and the B. Braun cell homogenizer (BH) were used to disrupt cells of Selenastrum capricornutum Printz for chlorophyll extraction. Cell disruption efficiency depended upon the number of cells filtered.
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Thomas Wiley Mill Model 4 GMITrusted Laboratory Solutions
Thomas Model 4 Wiley® Cutting Mill is ideal for the sample preparation of solid biofuels according to EN 1478. The Model 4 is a very popular mill for plant scientists and is a mill of choice. The Model 4 s grinding chamber is equipped with the right tools to satisfy any grinding procedure. The knives have specially formulated stainless
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Effect of the elapsed time between Wiley Online Library
· For glycoprotein release (also referred to as antigen retrieval) 40 mg of specimens were first homogenized in a Tapered Tissue Grinder (VWR Budapest Hungary) followed by the addition of 100 µL of RIPA buffer and 10 µL of 50 mM DTT to the homogenized samples and kept at 100˚C for 20 min then at 65˚C for 120 min.
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Mitochondria from rat liver Method Wiley Online
tissue grinder. Do not use a ground glass homogenizer. Fill the homogenizer to 1 inch (2.5 cm) below the flare with solution B and make three gentle passes by hand with the pestle in a slow downward rocking motion rather than twisting or grinding motion. A more vigorous machine driven homogenization may
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Bacteria detected in the honeybee Wiley Online Library
· Each sample was then washed three times with PBST and homogenized in 500 μl PBST using the Radnoti tissue grinder ( No. 440613 Monrovia CA). The total DNA was extracted with the Wizard ® Genomic DNA Purification kit (Promega Madison WI) according to
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Wiley Mini Mill Manualleibniz-festtage.de
Wiley Mill Price Natuurkundeproeven. Wiley mini mill manual.Minin wiley mill.Wiley mini mill plant grinder thomas wiley model 4 lab mill grinder the model 4 is a very popular mill for plant scientists and is a mill of choice wiley miller skier girlfriend prints mini cutting mills mill grinder price wiley miller skier mill.Read more thomas scientific wiley mill.
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Thomas Wiley Mills
The Wiley Mill by Thomas Scientific sets the standard of quality efficiency and value within the sample preparation market. With over 75 years of proven technology Wiley Mills are the most robust longest lasting mills on the market . The Wiley Mill is built by hand and consists of an all steel construction with four rotating steel cutting knives easy cleaning no service tech required
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Involvement of βγ Subunits of Gq/11 in Muscarinic M1
· The frontal lobes were homogenized using a Teflon-glass tissue grinder (12 up-and-down strokes by hand) in 10 volumes (wt/vol) of ice-cold buffer containing 10 mM HEPES-NaOH 1 mM EGTA 1 mM dithiothreitol and 1 mM MgCl 2 (pH 7.4). The homogenate was diluted with the same medium and centrifuged at 27 000 g for 20 min at 4°C. The pellet was
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Tissue Grinder at Thomas Scientific
Micro Tissue Grinder Kit DWK Life Sciences (Wheaton) Complete kit of six tissue grinders 0.1 0.2 1 1 2 and 1 mL Packed in a lightweight protective carrying case Glass-to-glass ground surface homogenizes connective tissue. Teflon ® units suitable for soft tissue.
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Tolerability and age-dependent toxicokinetics Wiley
· Fetuses were weighed and homogenized using a tissue grinder until a uniform homogenate was obtained. Stock solution of the internal standard methoxyurea was prepared in methanol at 1 mg/ml and diluted in acetonitrile to make a 10-μg/ml solution.
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Mitochondria from rat liver Method Wiley Online
tissue grinder. Do not use a ground glass homogenizer. Fill the homogenizer to 1 inch (2.5 cm) below the flare with solution B and make three gentle passes by hand with the pestle in a slow downward rocking motion rather than twisting or grinding motion. A more vigorous machine driven homogenization may
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Exercise increases MEF2‐ and GEF Wiley Online Library
· Muscle samples were homogenized with 10 passes of a Tenbroeck tissue grinder in 10 volumes of cytoplasmic extraction reagent I. Cytoplasmic extraction reagent II was added at a final concentration of 5 and the homogenate was spun in a centrifuge at 16 000 g for 5 min. The supernatant was extracted and the pellet was resuspended in 100 μl of
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Seasonal patterns of tissue biomass Wiley Online Library
was homogenized in a small glass tissue grinder (90 ﬁnal concentration) allowed to develop overnight at 2208C and centrifuged and absorbances were read on a spectrophotom-eter. Chl a content was calculated using equations of Jeffery and Humphrey (1975). Densities of symbiotic dinoﬂagellates
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Effects of propolis and gamma Wiley Online Library
Tissues were homogenized in a Dounce tissue grinder with RIPA buffer containing protease inhibitors phosphatase inhibitors PMSF and EDTA lysed on ice for 40 min and centrifuged at 4°C for 15 min. Western blotting analyses were performed with 30 μg of total protein as estimated by Bradford assays. Detection of signal was carried out with
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Short tail with skin lesion phenotype occurs in Wiley
in a prechilled tissue grinder. The samples were transferred in 0.5- to 1.0-ml cold lysis buffer into 1-ml glass tissue homogenators (Wheaton Science Prod-ucts Millville NJ USA) for homogenation. The lysates were sonicated for 10 s at output 20 (Soniﬁer 250
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Wiley Mini Mill Manualleibniz-festtage.de
Wiley Mill Price Natuurkundeproeven. Wiley mini mill manual.Minin wiley mill.Wiley mini mill plant grinder thomas wiley model 4 lab mill grinder the model 4 is a very popular mill for plant scientists and is a mill of choice wiley miller skier girlfriend prints mini cutting mills mill grinder price wiley miller skier mill.Read more thomas scientific wiley mill.
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Applications in Plant SciencesWiley
· The Geno/Grinder can also be used to process samples in 24–384 tubes or in deep-well titer plates. On the low end it is possible to modify a reciprocating saw for use as a tissue grinder (Alexander et al. 2006 Dean et al. 2018) for a fraction of the cost of commercially available machines. The grinding media used for the ball mills can
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